Microfluidic concentration of bacteria by on-chip electrophoresis

Microfluidic concentration of bacteria by on-chip electrophoresis.

In this contribution, we present a system for efficient preconcentration of pathogens without affecting their viability. Development of miniaturized molecular diagnostic kits requires concentration of the sample, molecule extraction, amplification, and detection. In consequence of low analyte concentrations in real-world samples, preconcentration is a critical step within this workflow. Bacteri...

Microfluidic Chip-capillary Electrophoresis with Ad- Justable On-chip Sample Dilution for Profiling of Urinary Markers

The paper reports the development of a microfluidic chip-capillary electrophoresis device using UV/Vis detection for profiling of urinary markers with adjustable on-chip sample dilution. A PMMA polymer microfluidic chip-CE device with on-chip ferrofluid valve control for adjustable sample dilution is developed for profiling of urinary markers related to metabolism disorders for clinical diagnos...

Sample concentration and impedance detection on a microfluidic polymer chip.

We present an on-chip microfluidic sample concentrator and detection triggering system for microparticles based on a combination of insulator-based dielectrophoresis (iDEP) and electrical impedance measurement. This platform operates by first using iDEP to selectively concentrate microparticles of interest based on their electrical and physiological characteristics in a primary fluidic channel;...

Antibody analysis by microfluidic on a chip

Analysis of microsatellite instability in colorectal carcinoma by microfluidic-based chip electrophoresis

Microsatellite analysis is an important tool in clinical research and molecular diagnostics because microsatellite instability (MSI) occurs frequently in various types of cancer. Approximately 10-15% of colorectal, gastric and endometrial carcinomas are associated with MSI, and this has an impact on clinical prognosis. The microsatellite loci Bat25, Bat26, D2S123, D5S346 and D17S250, recommende...